2BDV
X-Ray Crystal Structure of Phage-related Protein BB2244 from Bordetella bronchiseptica. Northeast Structural Genomics Consortium Target BoR24.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97932, 0.97905, 0.96767 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.954, 63.553, 80.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.220 - 2.200 |
| Rwork | 0.212 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.109 | 0.593 |
| Number of reflections | 16553 | |
| <I/σ(I)> | 21.3 | 3 |
| Completeness [%] | 95.7 | 71.6 |
| Redundancy | 12.3 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 10 mM Tris, 18% PEG3350, 200 mM Na Formate, 5 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.50 |
