2BDU
X-Ray Structure of a Cytosolic 5'-Nucleotidase III from Mus Musculus MM.158936
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97911 |
Spacegroup name | P 32 |
Unit cell lengths | 133.674, 133.674, 38.889 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.755 - 2.350 |
R-factor | 0.16588 |
Rwork | 0.163 |
R-free | 0.21980 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.548 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.755 | 2.400 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.091 | 0.367 |
Number of reflections | 31917 | |
<I/σ(I)> | 6.818 | 2.067 |
Completeness [%] | 98.5 | 87.8 |
Redundancy | 5.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 10 MG/ML PROTEIN, 26% PEG 8K, 0.10 M MOPS (HEPES USED AS CRYOPROTECTANT), pH 7.0, vapor diffusion, hanging drop, temperature 277K |