2BDP

CRYSTAL STRUCTURE OF BACILLUS DNA POLYMERASE I FRAGMENT COMPLEXED TO 9 BASE PAIRS OF DUPLEX DNA

Experimental procedure

Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1997-03-15
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	86.241, 93.275, 106.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.241
Rwork	0.241
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	UNPUBLISHED DNA COMPLEX STRUCTURE
RMSD bond length	0.010
RMSD bond angle	24.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.044 *	0.163 *
Total number of observations	253450 *
Number of reflections	73375
Completeness [%]	91.7	60.1
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	18 *	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	55 (%(v/v)sat)
3	1	reservoir	MES	100 (mM)
4	1	reservoir	MPD	2.5-7.0 (%(v/v))
5	1	reservoir	sodium azide	0.02 (%(w/v))