2BDE
Crystal Structure of the cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) from Legionella pneumophila, Northeast Structural Genomics Target LgR1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97898, 0.97943, 0.96795 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 152.540, 152.540, 188.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.120 - 2.900 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.26700 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.120 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.088 | 0.338 |
| Number of reflections | 44284 | |
| <I/σ(I)> | 29 | 10.93 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 24.9 | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 293 | 100mM MES, 20% PEG400, 100mM KBr, 5mM DTT, pH 6.15, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
