2BCY
Crystal Structure of a minimal, mutant all-RNA hairpin ribozyme (U39C, G8MTU)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 83 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.900, 93.900, 129.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.060 - 2.700 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.27000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ZFR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.780 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.058 | 0.457 |
| Number of reflections | 9556 | |
| <I/σ(I)> | 14.7 | 4 |
| Completeness [%] | 97.9 | 99.9 |
| Redundancy | 6.9 | 8.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | PEG2000 MME, lithium sulfate, spermidine, cobalt hexamine, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG2000 MME | ||
| 2 | 1 | 1 | lithium sulfate | ||
| 3 | 1 | 1 | spermidine | ||
| 4 | 1 | 1 | cobalt hexamine | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG2000 MME | ||
| 7 | 1 | 2 | lithium sulfate | ||
| 8 | 1 | 2 | cobalt hexamine |
