2BCO
X-ray structure of succinylglutamate desuccinalase from Vibrio Parahaemolyticus (RIMD 2210633) at the resolution 2.3 A, Northeast Structural Genomics Target Vpr14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.852, 72.555, 93.032 |
| Unit cell angles | 90.00, 105.66, 90.00 |
Refinement procedure
| Resolution | 30.260 - 2.330 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.043 | 0.175 |
| Number of reflections | 62939 | |
| <I/σ(I)> | 29.6 | 7.5 |
| Completeness [%] | 94.4 | 97.4 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG3350, 200 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
