2BBJ
Crystal structure of the CorA Mg2+ transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 136.979, 173.181, 134.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.900 |
| R-factor | 0.36301 |
| Rwork | 0.361 |
| R-free | 0.40649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bbh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.172 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.040 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.092 | 0.420 |
| Number of reflections | 28963 | |
| <I/σ(I)> | 13.3 | 1.23 |
| Completeness [%] | 95.9 | 77.2 |
| Redundancy | 11.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | 20% PEG2000, 0.3M Mg(NO3)2, 0.1M Tris, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | 20% PEG2000, 0.3M Mg(NO3)2, 0.1M Tris, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
