2BAZ

Structure of YosS, a putative dUTPase from Bacillus subtilis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-28
Detector	MARRESEARCH
Wavelength(s)	0.9803, 0.981, 1.1
Spacegroup name	P 43
Unit cell lengths	116.500, 116.500, 56.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.225
Rwork	0.217
R-free	0.24300
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.087	0.270
Number of reflections	34010
Completeness [%]	99.3	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	2.0M (NH4)2SO4, 0.1M Na Citrate, 0.2M K/Na Tartrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	2.0M (NH4)2SO4, 0.1M Na Citrate, 0.2M K/Na Tartrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	2.0M (NH4)2SO4, 0.1M Na Citrate, 0.2M K/Na Tartrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K