2BAA

THE REFINED CRYSTAL STRUCTURE OF AN ENDOCHITINASE FROM HORDEUM VULGARE L. SEEDS TO 1.8 ANGSTROMS RESOLUTION

Replaces:  1BAA

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	41.500, 64.500, 43.100
Unit cell angles	90.00, 108.00, 90.00

Refinement procedure

Resolution	5.000 - 1.800
R-factor	0.18 *
Rwork	0.180
RMSD bond length	0.017
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	3.880 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.077 *	0.209 *
Total number of observations	168007 *
Number of reflections	18975 *	1936 *
Completeness [%]	94.0 *	98 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	drop	Tris-HCl	100 (mM)
3	1	reservoir	ammonium sulfate	30 (%sat)