2BA2
Crystal structure of the DUF16 domain of MPN010 from Mycoplasma pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-01-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.559, 38.874, 68.734 |
| Unit cell angles | 90.00, 99.21, 90.00 |
Refinement procedure
| Resolution | 19.550 - 1.800 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.28100 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.010 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 32441 | |
| Completeness [%] | 80.4 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2M lithium Sulfate, 25% PEG3350, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
