2BA2
Crystal structure of the DUF16 domain of MPN010 from Mycoplasma pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2005-01-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97938 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.559, 38.874, 68.734 |
Unit cell angles | 90.00, 99.21, 90.00 |
Refinement procedure
Resolution | 19.550 - 1.800 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.28100 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.010 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 32441 | |
Completeness [%] | 80.4 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2M lithium Sulfate, 25% PEG3350, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |