2B9V
Acetobacter turbidans alpha-amino acid ester hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.369, 275.594, 197.953 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.20051 |
| Rwork | 0.199 |
| R-free | 0.23477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mpx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.663 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.341 |
| Number of reflections | 598744 | |
| Completeness [%] | 85.5 | 67.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 15-17% PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
