2B94
Structural analysis of P knowlesi homolog of P falciparum PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9184 |
Spacegroup name | H 3 2 |
Unit cell lengths | 98.377, 98.377, 160.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.990 - 1.850 |
R-factor | 0.18859 |
Rwork | 0.187 |
R-free | 0.22498 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb entries 1SQ6 1nw4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.152 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.169 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 24916 | |
<I/σ(I)> | 13 | 1.9 |
Completeness [%] | 96.9 | 81.4 |
Redundancy | 7.4 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | sodium thiosulfate, MOPS, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |