2B90

Crystal structure of the interleukin-4 variant T13DR85A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2004-08-19
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	91.330, 91.330, 45.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.21609
Rwork	0.214
R-free	0.24874
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hik
RMSD bond length	0.016
RMSD bond angle	1.661
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.700	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.055	0.258
Number of reflections	11729
<I/σ(I)>	18.2	5.6
Completeness [%]	99.4	98.7
Redundancy	5.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	52% Ammonium sulfate, 0.1M Sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K