2B81
Crystal Structure of the Luciferase-like Monooxygenase from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 138.466, 159.942, 172.914 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.810 - 2.500 |
| R-factor | 0.18619 |
| Rwork | 0.183 |
| R-free | 0.21833 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.364 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.810 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.140 | 0.766 |
| Number of reflections | 131719 | |
| <I/σ(I)> | 9.3 | 3.4 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | MES, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
