2B7U
Ribosome inactivating protein type 1 from Charybdis maritima AGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-02 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.240, 57.240, 51.088 |
| Unit cell angles | 90.00, 104.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | combined model from PARTS OF 12 RIP's |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.598 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.630 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.064 | 0.186 |
| Number of reflections | 35616 | |
| <I/σ(I)> | 18.1 | 5.9 |
| Completeness [%] | 97.0 | 82.2 |
| Redundancy | 4.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 283 | 100mM Mes, 16% PEG 6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
