2B7Q
Crystal structure of quinolinic acid phosphoribosyltransferase from Helicobacter pylori with nicotinate mononucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 148.855, 148.855, 145.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.950 - 3.310 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.800 |
| Data reduction software | AUTOMAR |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.950 | 20.000 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 24834 | |
| Completeness [%] | 91.3 | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 0.1M HEPES-NaOH, 1.5M Li2SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
