2B7K
Crystal Structure of Yeast Sco1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2003-04-07 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.700, 81.300, 79.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.620 - 1.800 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1WP0 MODIFIED VERSION |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.620 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 63909 | |
<I/σ(I)> | 4.8 | 2.5 |
Completeness [%] | 99.2 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.3 | 291 | Lithium Sulfate, Sodium Citrate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 5.30 |