2B7J
Crystal Structure of Yeast Sco1 with Copper Bound
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2005-03-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.600, 81.900, 79.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.650 - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b7k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 24962 | |
| <I/σ(I)> | 9.6 | 2.5 |
| Completeness [%] | 79.0 | 42.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 291 | Lithium Sulfate, Sodium Citrate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 5.30 |
