2B7C

Yeast guanine nucleotide exchange factor eEF1Balpha K205A mutant in complex with eEF1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-09
Detector	MARRESEARCH
Wavelength(s)	0.9778
Spacegroup name	P 21 21 21
Unit cell lengths	65.750, 93.530, 93.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.211
Rwork	0.210
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PBD entry 1F60
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.860
High resolution limit [Å]	1.790	1.790
Rmerge	0.029	0.231
Number of reflections	47973
<I/σ(I)>		3.4
Completeness [%]	88.6	69.9
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	277	12% PEG 2000 MME, 100mM Tris-Cl pH 7.6, 100mM KCl, 3mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 277K