2B74

T4 Lysozyme mutant L99A at 100 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	293
Detector technology	CCD
Collection date	2004-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9174
Spacegroup name	P 32 2 1
Unit cell lengths	60.755, 60.755, 96.052
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.630 - 2.100
R-factor	0.17473
Rwork	0.172
R-free	0.23190
Starting model (for MR)	1l90
RMSD bond length	0.019
RMSD bond angle	1.533
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.630	2.154
High resolution limit [Å]	2.100	2.100
Rmerge	0.052
Number of reflections	11258
Completeness [%]	95.0	98.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	~ 2.0 M Na/K phosphate, 50 mM beta-mercaptoethanol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K