2B6W

T4 Lysozyme mutant L99A at 200 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	293
Detector technology	CCD
Collection date	2004-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9174
Spacegroup name	P 32 2 1
Unit cell lengths	60.627, 60.627, 95.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.490 - 2.200
R-factor	0.1603
Rwork	0.158
R-free	0.20834
RMSD bond length	0.016
RMSD bond angle	1.398
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.490	2.259
High resolution limit [Å]	2.200	2.200
Rmerge	0.039
Number of reflections	9808
Completeness [%]	91.3	87.61

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	~2 M Na/K phosphate, 50 mM beta-mercaptoethanol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K