2B6T
T4 Lysozyme mutant L99A at 200 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2004-11-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9174 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.604, 60.604, 95.708 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.490 - 2.100 |
| R-factor | 0.15983 |
| Rwork | 0.158 |
| R-free | 0.19819 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l90 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.294 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.490 | 2.155 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.034 | |
| Number of reflections | 11469 | |
| Completeness [%] | 92.9 | 95.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ~2.0 Molar Na/K phosphates, 50 mM beta-mercaptoethanol, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293, temperature 20K |
