2B6P

X-ray structure of lens Aquaporin-0 (AQP0) (lens MIP) in an open pore state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 4 21 2
Unit cell lengths	109.531, 109.531, 52.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.880 - 2.400
Rwork	0.242
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Refinement software	CNS (1.1)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	300	20 mM glycine, pH 10.0 30% polyethylene glycol 1K 50 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 300K