2B6N
The 1.8 A crystal structure of a Proteinase K like enzyme from a psychrotroph Serratia species
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.430, 42.560, 74.260 |
| Unit cell angles | 90.00, 110.19, 90.00 |
Refinement procedure
| Resolution | 23.760 - 1.800 |
| R-factor | 0.163 |
| Rwork | 0.160 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ic6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.237 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.760 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.114 | 0.381 |
| Number of reflections | 23747 | |
| <I/σ(I)> | 6 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1M Tris, 45% ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
