2B61
Crystal Structure of Homoserine Transacetylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97880 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.264, 85.264, 120.263 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18700 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.155 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 57586 | |
| Completeness [%] | 98.8 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | PEG8000, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP |
