2B5U
Crystal Structure Of Colicin E3 V206C Mutant In Complex With Its Immunity Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-03 |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.730, 192.916, 85.385 |
| Unit cell angles | 90.00, 112.98, 90.00 |
Refinement procedure
| Resolution | 44.400 - 2.300 |
| R-factor | 0.291 |
| Rwork | 0.259 |
| R-free | 0.29500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jch |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.291 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.073 | 0.465 |
| Number of reflections | 88240 | |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 277 | Sodium citrate, pH 5.6, VAPOR DIFFUSION, temperature 277K |
