2B5N
Crystal Structure of the DDB1 BPB Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2004-03-06 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.543, 73.747, 136.778 |
| Unit cell angles | 90.00, 111.73, 90.00 |
Refinement procedure
| Resolution | 48.800 - 2.800 |
| R-factor | 0.2421 |
| Rwork | 0.236 |
| R-free | 0.29590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.800 |
| High resolution limit [Å] | 2.540 |
| Number of reflections | 62244 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 4000, iso-propanol, HEPES, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
