2B54

Human cyclin dependent kinase 2 (CKD2)complexed with DIN-232305

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-10-26
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	72.970, 73.160, 54.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.440 - 1.850
Rwork	0.158
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IN-HOUSE CDK2 MODEL
RMSD bond length	0.154
RMSD bond angle	9.500
Data reduction software	SCALE ((RIGAKU))
Data scaling software	SCALE ((RIGAKU))
Phasing software	X-PLOR
Refinement software	CNX (2002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.440	1.870
High resolution limit [Å]	1.840	1.840
Rmerge	0.099	0.457
Number of reflections	21779
<I/σ(I)>	3.1	0.5
Completeness [%]	83.7	67.3
Redundancy	2.2	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291	100mM HEPES pH 7.4, 50mM Ammonium Acetate, 2mM DTT, 4-14% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 291K