2B4W
Hypothetical protein from leishmania major
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-22 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97941, 0.97962, 0.95372 |
Spacegroup name | H 3 |
Unit cell lengths | 97.400, 97.400, 113.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.980 |
R-factor | 0.135 |
R-free | 0.21090 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.026 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.08) |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.054 | 0.131 |
Number of reflections | 28075 | |
<I/σ(I)> | 27.4 | 10.3 |
Completeness [%] | 92.8 | 30.1 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 277 | 0.4 ul protein 10.7 mg/ml, 0.4 ul crystallization buffer 100mM KH2PO4, 18% PEG PEG 4000,100 mM TAPS, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 9.00 |