2B4I
Crystal Structure of the Rhesus Rotavirus VP5 Antigen Domain Trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.123, 94.027, 97.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.2251 |
| Rwork | 0.222 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Residues 267-470 of VP5CT (1SLQ) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.497 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 55552 | |
| <I/σ(I)> | 26.85 | 4.25 |
| Completeness [%] | 95.7 | 97.2 |
| Redundancy | 4.71 | 4.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch | 8 | 281.15 | Tris, sodium chloride, EDTA, pH 8.0, Batch, temperature 281.15K |
