2B3V
Spermine spermidine acetyltransferase in complex with acetylcoa, K26R mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Wavelength(s) | 0.98 |
Spacegroup name | P 62 |
Unit cell lengths | 62.994, 62.994, 80.909 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.950 |
Rwork | 0.222 |
R-free | 0.25700 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.950 |
Number of reflections | 12884 |
Completeness [%] | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | mmPEG5000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |