2B3R

Crystal structure of the C2 domain of class II phosphatidylinositide 3-kinase C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	53.365, 53.365, 200.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 2.300
R-factor	0.197
Rwork	0.197
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	13692
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	PEG 6K, Li2SO4, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	PEG 6K, Li2SO4, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	PEG 6K, Li2SO4, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K