2B3L
Crystal structure of type I human methionine aminopeptidase in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.217, 77.276, 47.850 |
| Unit cell angles | 90.00, 91.59, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.111 |
| Rwork | 0.117 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yj3 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.031 | 0.128 |
| Number of reflections | 51841 | |
| Completeness [%] | 95.1 | 83.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG 2000, potassium chloride, hepes, sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
