2B3F
Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 121.376, 134.958, 159.719 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.140 - 1.560 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20500 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.000 | 1.630 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.061 | 0.439 |
| Number of reflections | 352612 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 95.6 | 64 |
| Redundancy | 6.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | 10% (w/v) Isopropanol, 0.1M Sodium Citrate pH5.6, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
