2B33
CRYSTAL STRUCTURE OF A PUTATIVE ENDORIBONUCLEASE (TM0215) FROM THERMOTOGA MARITIMA MSB8 AT 2.30 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 53.280, 53.280, 228.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.640 - 2.300 |
| Rwork | 0.199 |
| R-free | 0.26100 |
| Structure solution method | MR |
| Starting model (for MR) | 1qahA |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.412 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.640 | 2.200 | |
| High resolution limit [Å] | 2.120 | 4.550 | 2.120 |
| Rmerge | 0.218 | 0.901 | 0.901 |
| Number of reflections | 13161 | 2682 | 1964 |
| <I/σ(I)> | 5.27 | 12.29 | 1.76 |
| Completeness [%] | 80.9 | ||
| Redundancy | 2.2 | 1.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.3 | 293 | 0.2 M Ca Acetate, 20.0% PEG 3350, No Buffer, pH 7.3, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
