2B31

Crystal structure of the complex formed between goat signalling protein with pentasaccharide at 3.1 A resolution reveals large scale conformational changes in the residues of TIM barrel

Replaces:  1ZU9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2005-05-14
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	62.745, 66.613, 107.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.700 - 3.100
R-factor	0.17859
Rwork	0.177
R-free	0.21782
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1QZO
RMSD bond length	0.020
RMSD bond angle	2.440
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.700	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	8493
<I/σ(I)>	7	2.4
Completeness [%]	98.5	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.8	298	25mM Tris HCl, 50mM NaCl, 19% ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0 K, pH 7.80