2B2N
Structure of transcription-repair coupling factor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97788, 0.9798, 0.97395 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 112.650, 112.650, 213.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| Rwork | 0.198 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.376 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 47377 | |
| Completeness [%] | 99.5 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | PEG 4000, sodium citrate, ammonium sulfate, sodium formiate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
