2B2G
MS2 Wild-type RNA stemloop complexed with an N87S mutant MS2 capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2001-11-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 287.450, 287.450, 651.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.020 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.020 |
| Number of reflections | 162584 | |
| <I/σ(I)> | 8.2 | 1.9 |
| Completeness [%] | 80.7 | 44.4 |
| Redundancy | 1.6 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 303 | MS2 COATPROTEIN in 1.25% or 1.5% PEG 8000, 0.4M SODIUM PHOSPHATE BUFFER, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | 0.4M SODIUM PHOSPHATE BUFFER | ||
| 3 | 1 | 1 | water | ||
| 4 | 1 | 2 | PEG 8000 | ||
| 5 | 1 | 2 | 0.4M SODIUM PHOSPHATE BUFFER | ||
| 6 | 1 | 2 | water |
