2B2C

Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	75.15
Detector technology	CCD
Collection date	2005-02-05
Detector	MARRESEARCH
Wavelength(s)	0.89978
Spacegroup name	P 1 21 1
Unit cell lengths	59.990, 99.230, 67.850
Unit cell angles	90.00, 107.20, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.22032
Rwork	0.219
R-free	0.25415
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.484
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.108
Number of reflections	25260
Completeness [%]	94.9	88.77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	PEG 3350, Lithium sulfate, Tris-CL, Dithiothreitol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K