2B2C
Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 75.15 |
| Detector technology | CCD |
| Collection date | 2005-02-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.89978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.990, 99.230, 67.850 |
| Unit cell angles | 90.00, 107.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.22032 |
| Rwork | 0.219 |
| R-free | 0.25415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.108 | |
| Number of reflections | 25260 | |
| Completeness [%] | 94.9 | 88.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | PEG 3350, Lithium sulfate, Tris-CL, Dithiothreitol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
