2B21
RADA Recombinase in complex with AMPPNP at pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-16 |
| Detector | ENRAF-NONIUS |
| Spacegroup name | P 61 |
| Unit cell lengths | 83.071, 83.071, 107.168 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.215 |
| Rwork | 0.214 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t4g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.270 |
| Data reduction software | LSCALE |
| Data scaling software | LSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.081 | 0.417 |
| Number of reflections | 16344 | |
| <I/σ(I)> | 8.1 | 1.8 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | VAPOR DIFFUSION, HANGING DROP, temperature 294K, pH 6.0 |
