2B1N
Crystal structure of a papain-fold protein without the catalytic cysteine from seeds of Pachyrhizus erosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.358, 61.358, 143.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.350 - 2.400 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.26700 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 5.900 | 2.400 |
| Rmerge | 0.080 | 0.033 | 0.504 |
| Number of reflections | 11433 | ||
| Completeness [%] | 99.8 | ||
| Redundancy | 8.8 | 8.1 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 2.0M ammonium sulfate, 5% isopropyl alcohol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
