2B1M
Crystal structure of a papain-fold protein without the catalytic cysteine from seeds of Pachyrhizus erosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.518, 61.518, 142.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.120 - 2.000 |
| Rwork | 0.180 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 4.930 | 2.000 |
| Rmerge | 0.071 | 0.034 | 0.386 |
| Number of reflections | 19291 | ||
| Completeness [%] | 99.9 | ||
| Redundancy | 12.4 | 12.4 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
