2B1A
Crystal structure analysis of anti-HIV-1 V3 Fab 2219 in complex with UG1033 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98397 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.716, 96.890, 97.018 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.310 - 2.348 |
| R-factor | 0.21287 |
| Rwork | 0.211 |
| R-free | 0.24446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b0s |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.328 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.310 | 2.390 |
| High resolution limit [Å] | 2.348 | 2.348 |
| Number of reflections | 24986 | |
| <I/σ(I)> | 31.9 | 2.36 |
| Completeness [%] | 98.6 | 92.8 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 13.75% PEG 20000, 0.05M disodium tartrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
