2B13
Truncated S. aureus LytM, P41 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 65.962, 65.962, 62.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.800 - 1.550 |
| R-factor | 0.17303 |
| Rwork | 0.172 |
| R-free | 0.20054 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qwy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.480 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.800 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.037 | 0.152 |
| Number of reflections | 38546 | |
| <I/σ(I)> | 25.8 | 10 |
| Completeness [%] | 96.3 | 86.1 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 30 % PEG 8000, 0.2 M L(+)-tartrate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
