2B0P
truncated S. aureus LytM, P212121 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.093, 78.261, 102.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 1.500 |
| R-factor | 0.18241 |
| Rwork | 0.180 |
| R-free | 0.22239 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qwy |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.703 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.068 | 0.256 |
| Number of reflections | 42130 | |
| <I/σ(I)> | 6.4 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 294 | 0.2 M ammonium sulfate, 0.1 M sodium cacodylate, 0.1 M sodium acetate, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
