2B0L

C-terminal DNA binding domain of transcriptional pleiotropic repressor CodY.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2005-08-12
Detector	MARRESEARCH
Wavelength(s)	1.54178
Spacegroup name	P 4 2 2
Unit cell lengths	68.128, 68.128, 164.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.20445
Rwork	0.202
R-free	0.25033
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.544
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	HKL2Map
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.974
High resolution limit [Å]	2.900	2.900
Rmerge	0.072	0.655
Number of reflections	8705
<I/σ(I)>	15.4	1.2
Completeness [%]	94.4	84.3
Redundancy	3.7	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	ammoium sulphate, sodium citrate, NaCl, glycerol, tacsimate, TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	ammoium sulphate, sodium citrate, NaCl, glycerol, tacsimate, TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	ammoium sulphate, sodium citrate, NaCl, glycerol, tacsimate, TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K