2B0J
The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (Hmd)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97858 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.470, 59.880, 68.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.750 |
| Rwork | 0.191 |
| R-free | 0.21679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | structure of Hmd from Methanopyrus kandleri |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.540 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.795 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.075 | 0.640 |
| Number of reflections | 38541 | |
| <I/σ(I)> | 14.1 | 2.5 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | Hepes/NaOH, 2-propanol, PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
