2B0D
EcoRV Restriction Endonuclease/GAATTC/Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-01 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.000, 48.400, 63.600 |
| Unit cell angles | 97.20, 109.00, 106.60 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| Rwork | 0.220 |
| R-free | 0.30200 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.920 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.100 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 30291 | |
| <I/σ(I)> | 8 | 3.1 |
| Completeness [%] | 89.1 | 82.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | PEG 4K, Hepes, NaCl, CaCl2, Glycerol, pH 7.5, vapor diffusion, hanging drop, temperature 297K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4K | ||
| 10 | 1 | 2 | Glycerol | ||
| 11 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Hepes | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | CaCl2 | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 4K | ||
| 7 | 1 | 2 | Hepes | ||
| 8 | 1 | 2 | NaCl | ||
| 9 | 1 | 2 | CaCl2 |
