2B0C
The crystal structure of the putative phosphatase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.256, 62.256, 127.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.20337 |
| Rwork | 0.201 |
| R-free | 0.25880 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.273 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.049 | 0.578 |
| Number of reflections | 32006 | |
| <I/σ(I)> | 56.5 | 2.35 |
| Completeness [%] | 99.2 | 93.2 |
| Redundancy | 14.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.05M MES, 25% PEG 5KMME, 0.2M (NH4)2SO4, 10mM MgCl2, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
