2B06
Crystal structure of the MutT/nudix family protein from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.850, 64.990, 41.779 |
| Unit cell angles | 90.00, 127.60, 90.00 |
Refinement procedure
| Resolution | 40.690 - 1.400 |
| R-factor | 0.19046 |
| Rwork | 0.189 |
| R-free | 0.21923 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.215 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.045 | 0.157 |
| Number of reflections | 25116 | |
| <I/σ(I)> | 22.8 | 3.3 |
| Completeness [%] | 94.5 | 79.5 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1M Tris/HCl, 0.2M MgSO4, 25% PEG3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
